Regulatory
pLux/Tet

Part:BBa_K176000

Designed by: Danqian Liu, Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-05-06)

pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS

sense two inputs, activated by LuxR/3OC6HSL and repressed by tetR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

MEASUREMENT

K176000, the hybrid promoter，is the heart of our project. To investigate the response of K176000, we performed a lot of measurement by building different devices. At last, we obtain a lot of data which can show the response of K176000 to time, AHL, aTc and tetR.

To see the detail data of each measurement device, please click the link after each biobrick number.

AHL&time-->K176000

K176026[1]: pCon 0.70->luxR+pLux/Tet->GFP

K176126[2]: pCon 0.36->luxR+pLux/Tet->GFP

K176128[3]: pCon 0.15->luxR+pLux/Tet->GFP

K176130[4]: pCon 0.04->luxR+pLux/Tet->GFP

AHL&aTc-->K176000

K176071[5]: pCon 0.36->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP

K176075[6]: pCon 0.15->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFP

K176078[7]: pCon 0.01->tetR+pCon 0.70->luxR+pLux/Tet->GFP

AHL&tetR-->K176000

K176136[8]: pCon 0.70->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP

K176140[9]: pCon 0.15->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP

K176144[10]: pCon 0.01->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFP



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Categories
Parameters
n/apLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS